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    Structured Review

    Alomone Labs acc
    Acc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cav3+1/Anti-CACNA1G+(CaV3%2E1)+Antibody/pm42025169-537-26-24
    Average 94 stars, based on 53 article reviews
    acc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels
    Article Snippet: Briefly, samples containing 20 μg of protein were loaded and separated by 10% (for the Cav3.1, Cav3.2, Cav3.3 and GR proteins) or 12.5% (for the H3K9me2 and H3K27me3 proteins) SDS-polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After blocking with 5% skimmed milk for 1 h, the membranes were incubated overnight at 4 °C with primary antibodies against the following: Cav3.1 (rabbit, 1:1000, Alomone Labs), Cav3.2 (rabbit, 1:1000, Alomone Labs), Cav3.3 (rabbit, 1:1000, Alomone Labs), GR (rabbit, 1: 1000, Abcam), H3K9me2 (mouse, 1:1000, Abcam), H3K27me3 (mouse, 1:1000, Abcam), H3 (rabbit, 1:1000, Abcam) and GAPDH (rabbit, 1:5000, Abcam). .. After the primary antibody had been removed, the membrane was washed three times with TBST, followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit (1:8000, R&D Systems) or goat anti-mouse secondary antibody (1:8000, R&D Systems) for 1 h at room temperature.

    Article Title: Endothelin 3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration
    Article Snippet: .. After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged. ..

    Article Title: Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration.
    Article Snippet: .. After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged. ..

    Article Title: Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration
    Article Snippet: .. After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24 h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged. ..

    Incubation:

    Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels
    Article Snippet: Briefly, samples containing 20 μg of protein were loaded and separated by 10% (for the Cav3.1, Cav3.2, Cav3.3 and GR proteins) or 12.5% (for the H3K9me2 and H3K27me3 proteins) SDS-polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After blocking with 5% skimmed milk for 1 h, the membranes were incubated overnight at 4 °C with primary antibodies against the following: Cav3.1 (rabbit, 1:1000, Alomone Labs), Cav3.2 (rabbit, 1:1000, Alomone Labs), Cav3.3 (rabbit, 1:1000, Alomone Labs), GR (rabbit, 1: 1000, Abcam), H3K9me2 (mouse, 1:1000, Abcam), H3K27me3 (mouse, 1:1000, Abcam), H3 (rabbit, 1:1000, Abcam) and GAPDH (rabbit, 1:5000, Abcam). .. After the primary antibody had been removed, the membrane was washed three times with TBST, followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit (1:8000, R&D Systems) or goat anti-mouse secondary antibody (1:8000, R&D Systems) for 1 h at room temperature.

    Article Title: Connexin 43 participates in atrial electrical remodelling through colocalization with calcium channels in atrial myocytes.
    Article Snippet: Atrial fibrillation (AF) is a highly prevalent cardiac arrhythmia and is associated with increased morbidity and mortality.1 There are many limitations in the current treatment of AF, including significant adverse effects for antiarrhythmic drugs and recurrences for catheter treatment.. Therefore, a better understanding of the mechanisms underlying AF is needed to improve treatment strategies.. Ca2+ overload is believed to play an important role in atrial electrical remodelling caused by tachycardia.

    Article Title: Endothelin 3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration
    Article Snippet: .. After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged. ..

    Article Title: Sex and age affect depot expression of Ca 2+ channels in rat white fat adipocytes
    Article Snippet: .. Proteins were detected by overnight incubation at 4 °C with primary antibodies anti CaV3.1 (Alomone ACC021) at a 1:500 dilution, anti Na,K-ATPase at 1:20,000 (Abcam ab76020)and anti β-actin (Sigma A1978) at 1:5000 dilution. .. Proteins were detected by overnight incubation at 4 °C with primary antibodies anti CaV3.1 (Alomone ACC021) at a 1:500 dilution, anti Na,K-ATPase at 1:20,000 (Abcam ab76020)and anti β-actin (Sigma A1978) at 1:5000 dilution.

    Article Title: Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration.
    Article Snippet: .. After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged. ..

    Article Title: Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration
    Article Snippet: .. After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24 h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged. ..

    Saline:

    Article Title: Connexin 43 participates in atrial electrical remodelling through colocalization with calcium channels in atrial myocytes.
    Article Snippet: Atrial fibrillation (AF) is a highly prevalent cardiac arrhythmia and is associated with increased morbidity and mortality.1 There are many limitations in the current treatment of AF, including significant adverse effects for antiarrhythmic drugs and recurrences for catheter treatment.. Therefore, a better understanding of the mechanisms underlying AF is needed to improve treatment strategies.. Ca2+ overload is believed to play an important role in atrial electrical remodelling caused by tachycardia.

    Cell-Signaling:

    Article Title: Connexin 43 participates in atrial electrical remodelling through colocalization with calcium channels in atrial myocytes.
    Article Snippet: Atrial fibrillation (AF) is a highly prevalent cardiac arrhythmia and is associated with increased morbidity and mortality.1 There are many limitations in the current treatment of AF, including significant adverse effects for antiarrhythmic drugs and recurrences for catheter treatment.. Therefore, a better understanding of the mechanisms underlying AF is needed to improve treatment strategies.. Ca2+ overload is believed to play an important role in atrial electrical remodelling caused by tachycardia.

    Staining:

    Article Title: Contribution of T-type calcium channel isoforms to cold and mechanical sensitivity in naïve and oxaliplatin-treated mice of both sexes.
    Article Snippet: .. Sections were stained using Cav3.1 (Alomone Labs Cat# ACC-021, RRID:AB_ 2039779; 1:500), c-fos (Abcam Cat# ab190289, RRID:AB_2737414; 1:500) and DAPI (Thermo Fisher Scientific Cat# D1306, RRID:AB_ 2629482; 1:1000). .. Stained sections were imaged using a confocal Leica microscope.



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